Isolation and culture of adult mouse vestibular nucleus neurons

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Isolation and culture of adult mouse vestibular nucleus neurons

Background/aim: Isolated cell cultures are widely used to study neuronal properties due to their advantages. Although embryonic animals are preferred for culturing, their morphological or electrophysiological properties may not reflect adult neurons, which may be important in neurodegenerative diseases. This paper aims to develop a method for preparing isolated cell cultures of medial vestibula...

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Culture of adult mouse neurons.

Primary neuronal cells used to model physiology are generally limited to embryonic tissue. However, embryonic tissue is not optimal as a model for age-related changes in physiology or late-onset disease. Successful culturing of neurons from adult animals, however, has been historically difficult, if not impossible. Here, we report methodology for routine and reliable cultivation of healthy stri...

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Physiological and anatomical properties of mouse medial vestibular nucleus neurons projecting to the oculomotor nucleus.

Neurons in the medial vestibular nucleus (MVN) vary in their projection patterns, responses to head movement, and intrinsic firing properties. To establish whether neurons that participate in the vestibulo-ocular reflex (VOR) have distinct intrinsic physiological properties, oculomotor nucleus (OMN)-projecting neurons were identified in mouse brainstem slices by fluorescent retrograde labeling ...

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Isolation and culture of adult rat hippocampal neurons.

Inability to culture adult central neurons and the failure of injured neurons to regenerate in the brain could be due to genetic controls or environmental inhibitors. We tested the environmental inhibitor hypothesis by attempting to regenerate adult rat neurons in B27/Neurobasal culture medium, a medium optimized for survival of embryonic neurons. To isolate neurons from their numerous connecti...

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ژورنال

عنوان ژورنال: TURKISH JOURNAL OF MEDICAL SCIENCES

سال: 2017

ISSN: 1300-0144,1303-6165

DOI: 10.3906/sag-1706-158